Clc genomics workbench 8.5 exclude host using primers

#CLC GENOMICS WORKBENCH 8.5 EXCLUDE HOST USING PRIMERS FREE#

The gut microbiome might be one of the important factors in the prevalence of centenarians in these regions. Although a previous study has reported the differences in the gut microbiota in these regions compared with those in urbanized towns, the gut microbiota of the local centenarians were not analyzed. The neighboring counties of Gurye, Gokseong, Sunchang, and Damyang, located in the southwestern part of Korea, are reported to form a longevity belt with a high prevalence of centenarians (KOSIS, 2017). This information and the potential modulation of gut microbiota can improve the health of the elderly. Therefore, there is a need to analyze gut microbiota of centenarians and collect the observations in a public database. In addition, gut microbiota analyzed in previous studies (8 in China, 7 in Japan, and 39 in Italy) could be insufficient to characterize the gut microbiota in centenarians.

These discrepancies could be due to the differences in diet, lifestyle, genetic factors, and analysis methods. Although similar results of gut microbiota with aging were observed in previous studies, there were differences regarding the precise changes of gut microbiota. However, the gut microbiota in centenarians has higher bacterial diversity, which is likely linked to good immunological and metabolic health. The gut microbiota of the elderly has been associated with the host immune homeostasis via inflammation and can contribute to the progression of diseases and frailty in the elderly. Studies reported that with aging there was a reduction of bacterial diversity and increased colonization of opportunistic pathogens, such as Staphylococcaceae, Enterobacteriaceae, and Enterococcaceae, along with a reduction of beneficial microbes such as short-chain fatty acid (SCFA)-producing bacteria. The possible determination of healthy aging by gut microbiome was proposed due to its effects on human metabolism and immune system. Association of the human gut microbiome with metabolic disorder, obesity, inflammatory bowel disease, and infections has also been analyzed. Recently, several studies revealed that the human gut microbiome shows differences based on the age, place of residence, and diet of different individuals. However, information on longevity factors is limited and further studies are necessary to find such longevity attributes.

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Because of the suppressed mortality and morbidity, centenarians have been regarded as a model for healthy aging and some studies have been performed to find the genetic and/or environmental factors that might play a role in healthy and disease free aging. These individuals have been reported to present lower incidence of chronic illness, reduced morbidity and an extended healthy lifespan. KeywordsД¬entenarians are individuals of exceptionally advanced age. Although further studies are necessary to validate the function of microbiota between groups, this study provides valuable information on centenariansвЂ™ gut microbiota. These three metabolic pathways of gut microbiota can be associated with the immune status and healthy gut environment of centenarians. In addition, phosphatidylinositol signaling system, glycosphingolipid biosynthesis, and various types of N-glycan biosynthesis were predicted to be higher in the gut microbiota of centenarians (corrected p < 0.05). These differences could be due to differences in diet patterns and living environments. Gut microbiota of centenarians in rehabilitation hospital were also different to those residing at home. Age-related rearrangement of gut microbiota was observed in centenarians, such as reduced proportions of Faecalibacterium and Prevotella, and increased proportion of Escherichia, along with higher abundances of Akkermansia, Clostridium, Collinsella, and uncultured Christensenellaceae. The relative abundance of Firmicutes was found to be considerably higher in subjects from longevity villages than those from urbanized towns, whereas Bacteroidetes was lower.

Fecal samples were collected from centenarians, elderly, and young adults in longevity villages, and the gut microbiota sequences of elderly and young adults in urbanized towns of Korea were obtained from public databases. In this study, we analyzed and compared the gut microbiota of centenarians in longevity villages with the elderly and adults in the same region and urbanized towns. Several studies have attempted to identify factors associated with longevity and maintenance of health in centenarians.

Clc genomics workbench 8.5 exclude host using primers